Gene expression of MTOR, MURF-1 and MAFBX in wistar rats supplemented with whey proteins

  • Marcos Roberto Campos de Macêdo Programa de Pós-Graduação em Saúde do Adulto-PPGSAD na Universidade Federal do Maranhão-UFMA, Brasil.
  • Antonio Coppi Navarro Programa de Pós-Graduação em Educação Física na Universidade Federal do Maranhão-PPGEF/UFMA, Brasil.
  • Raphael Furtado Marques Programa de Pós-Graduação em Educação Física na Universidade Federal do Maranhão-PPGEF/UFMA, Brasil.
  • Melaine Mont´Alverne Lawall Silva Programa de Pós-Graduação em Saúde do Adulto-PPGSAD na Universidade Federal do Maranhão-UFMA, Brasil.
  • Flavia Castello Branco Vidal Programa de Pós-Graduação em Saúde do Adulto-PPGSAD na Universidade Federal do Maranhão-UFMA, Brasil.
  • Alanna Joselle Santiago Silva Programa de Pós-Graduação em Saúde do Adulto-PPGSAD na Universidade Federal do Maranhão-UFMA, Brasil.
  • Carlos Eduardo Neves Amorim Programa de Pós-Graduação em Educação Física na Universidade Federal do Maranhão-PPGEF/UFMA, Brasil.
  • Francisco Navarro Programa de Pós-Graduação em Educação Física na Universidade Federal do Maranhão-PPGEF/UFMA, Brasil.
Keywords: Whey Proteins, MTOR, MURF-1, MAFBX, Gastrocnemius

Abstract

To verify the effect of supplementation of different doses of Whey Proteins for 12 weeks on the gene expression of MTOR, MURF-1, MAFBX and on the alteration of the cellular diameter of the gastrocnemius muscle. Sample: 38 Wistar rats and supplemented for 12 weeks. The white portion of the right gastrocnemius muscle was embedded in Rna Latter solution and stored at -80 ° C and subjected to real-time polymerase chain reaction analysis. The left gastrocnemius muscle was intended for histological analysis. Gene expression of MTOR mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than in the control group, in the order of 100%, 97%, 96%, respectively. Likewise, the gene expression of MURF-1 mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than the control group, in the order of 65%, 75%, 84%, respectively. In line, the gene expression MAFBX mRNA had lower statistical significance in the groups Whey 2, Whey 4, Whey 6 in relation to the control group, in the order of 99%, 97%, 99%, respectively. There was no statistical difference in the cellular area of the gastrocnemius muscle between the groups. Therefore, supplementation of Whey Proteins at doses of 2, 4, 6 g / kg / day for 12 weeks in sedentary animals was not able to increase the gene expression of MTOR mRNA, however, it was able to reduce the gene expression of MURF -1 mRNA, MAFBX mRNA.

References

-Bacurau, R.F. Nutrição e suplementação esportiva. São Paulo. Phorte. p.73-91. 2009.

-Batistela, E.; Pereira, M.P.; Siqueira, J.T.; Paula-Gomes, S.; Zanon, N.M.; Oliveira, E.B.; Navegantes, L.C.C.; Kettelhut, I.C.; Andrade, C.M.B.; Kawashita, N.H.; Baviera, A.M. Decreased rate of protein synthesis, caspase-3 activity, and ubiquitin–proteasome proteolysis in soleus muscles from growing rats fed a low-protein, high-carbohydrate diet. Can. J. Physiol. Pharmacol. Vol. 92. p. 445-454. 2014.

-Brasnutri. Panorama do Setor. (2016). Acessado em 16/10/2017:< http://www.brasnutri.org.br/arquivos/numeros_setor/2017_atualizado.pdf >.

-Bodine, S.C.; Baar, K. Analysis of skeletal muscle hypertrophy in models of increased loading. Methods Mol. Biol. Vol. 798. p.213-229. 2012.

-Haraguchi, F.K.; Magalhaes, C.L.B.; Neves, L.X.; Santos, R.C.; Pedrosa, M.L.; Silva, M.E. Whey protein modifies gene expression related to protein metabolism affecting muscle weight in resistance-exercised rats. Nutrition. Vol. 30. p.876-881. 2014.

-IOM. Institute of Medicine. Dietary Reference Intakes: The Essential Guide to Nutrient Requirements. Washington, DC: The National Academies Press. 2006.

-Krissansen, G.W. Emerging Health Properties of Whey Proteins and Their Clinical Implications. Journal of the American College of Nutrition, 26, 713S-723S. 2007.

-Lancha Junior, A.R.; Campos-Ferraz, P.L.; Rogeri, P.S. Suplementação Nutricional no Esporte. Rio de Janeiro. Guanabara Koogan. Capítulo 8. p.135-166. 2009.

-Leary, S.; Underwood, W.; Anthony, R.; Cartner, S.; Corey, D.; Grandin, T.; Greenacre, C.; Gwaltney-Brant, S.; Mccrackin, M.A.; Meyer, R.; Miller, D.; Shearer, J.; Yanong, R. AVMA Guidelines for the Euthanasia of Animals. American Veterinary Medical Association. 2013.

-Lomonosova, Y.N.; Shenkman, B.S.; Kalamkarov, G.R.; Kostrominova, T.Y.; Nemirovskaya, T.L. L-arginine Supplementation Protects Exercise Performance and Structural Integrity of Muscle Fibers after a Single Bout of Eccentric Exercise in Rats. PLoS ONE. Vol. 9. p.e94448. 2014.

-Lomonosova, Y.N.; Kalamkarov, G.R.; Bugrova, A.E.; Shevchenko, T.F.; Kartashkina, N.L.; Lysenko, E.A.; Shvets, V.I.; Nemirovskaya, T.L. Protective Effect of L_Arginine Administration on Proteins of Unloaded m. soleus. Biochemistry. Vol. 76. p.571-580. 2011.

-Luo, J.; Chen, D.; Yu, B. Effects of different dietary protein sources on expression of genes related to protein metabolism in growing rats. British Journal of Nutrition. Vol. 104. p. 1421-1428. 2010.

-Mobley, C.B.; Hornberger, T.A.; Fox, C.D.; Healy, J.C.; Ferguson, B.S.; Lowery, R.P.; Mcnally, R.M.; Lockwood, C.M.; Stout, J.R.; Kavazis, A.N.; Wilson, J.M.; Roberts, M.D. Effects of oral phosphatidic acid feeding with or without Whey protein on muscle protein synthesis and anabolic signaling in rodent skeletal muscle. Journal of the International Society of Sports Nutrition. Vol. 12. 2015.

-Neves, S. M. P. Manual de cuidados e procedimentos com animais de laboratório do Biotério de Produção e Experimentação da FCF-IQ. São Paulo: FCF-IQ/USP. 216 p. 2013.

-Xia, Z.; Cholewa, J.; Zhao, Y.; Yang, Y.Q.; Shang, H.Y.; Guimarães-Ferreira, L.; Naimo, M.A.; Su, Q.S.; Zanchi, N.E. Hypertrophy-Promoting Effects of Leucine Supplementation and Moderate Intensity Aerobic Exercise in Pre-Senescent Mice. Nutrients. Vol. 2. p. 246. 2016.

-WHO. Organização Mundial de Saúde. FAO/WHO iniciam um relatório pericial sobre dieta alimentar, nutrição e prevenção de doenças crónicas. OMS. 2003.

Published
2023-01-30
How to Cite
Macêdo, M. R. C. de, Navarro, A. C., Marques, R. F., Silva, M. M. L., Vidal, F. C. B., Silva, A. J. S., Amorim, C. E. N., & Navarro, F. (2023). Gene expression of MTOR, MURF-1 and MAFBX in wistar rats supplemented with whey proteins. Brazilian Journal of Obesity, Nutrition and Weight Loss, 16(104), 907-917. Retrieved from https://www.rbone.com.br/index.php/rbone/article/view/2151
Issue
Vol. 16 No. 104 (2022)
Section
Scientific Articles - Original

Copyright (c) 2023 Marcos Roberto Campos de Macêdo, Antonio Coppi Navarro, Raphael Furtado Marques, Melaine Mont´Alverne Lawall Silva, Flavia Castello Branco Vidal, Alanna Joselle Santiago Silva, Carlos Eduardo Neves Amorim, Francisco Navarro

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish in this journal agree to the following terms:

  • Authors retain the copyright and grant the journal the right of first publication, with work simultaneously licensed under the Creative Commons Attribution License BY-NC which allows the sharing of the work with acknowledgment of the authorship of the work and initial publication in this journal.
  • Authors are authorized to enter into additional contracts separately for non-exclusive distribution of the version of the work published in this journal (eg, publishing in institutional repository or book chapter), with acknowledgment of authorship and initial publication in this journal.
  • Authors are allowed and encouraged to post and distribute their work online (eg, in institutional repositories or on their personal page) at any point before or during the editorial process, as this can bring about productive change as well as increase impact and impact. citation of published work (See The Effect of Free Access).